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KMID : 1102020180480010001
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Applied Microscopy
2018 Volume.48 No. 1 p.1 ~ p.5
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Image Processing and Cryo-Transmission Electron Microscopy; Example of Human Proteasome
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Choi Hyo-Sun
Jeon Hyun-Bum
Noh Seul-Gi
Kwon Oh-Kyung
Mun Ji-Young
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Abstract
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Cryo-transmission electron microscopy (cryo-TEM) allows us to perform structural analysis of a analyses of large protein complexes, which are dif?cult to analyze using X-ray crystallography or nuclear magnetic resonance. The most common examples of proteins used are ribosomes and proteasomes. In this paper, we brie?y describe the advantage of cryo-TEM and the process of two-dimensional classification by considering a human proteasome as an example.
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KEYWORD
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Cryo-transmission electron microscopy, Proteasome, Two-dimensional classi-cation, Negative staining
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